Lab resource

Multi-functional optical imaging system (다기능성 광학 이미징 시스템)

STORM

이미징 모드 (Imaging mode)

  • 광시야 형광 현미경, Conventional Fluorescence Microscopy
  • 전반사 형광 현미경, Total Internal Reflection (TIRF) Fluorescence Microscopy
  • 단분자 현미경, Single Molecule Localization Microscopy (SMLM)
    – STORM: 25/50 nm (횡방향/종방향 분해능) for organic dyes
    – PALM: 40/100 nm (횡방향/종방향 분해능) for fluorescent proteins
    – PAINT (Point Accumulation for Imaging in Nanoscale Topography)

사양 (Specification)

  • Microscope platform: Nikon’s Ti2-E
  • Laser lines: 405 nm, 488 nm, 560 nm, 642 nm
  • Objectives: 100x/1.45 (oil), 100x/1.49 (oil, TIRF), 100x/1.35 (silicone oil), 60x/1.27 water, 20x/0.75
  • Cameras: Andor’s EMCCD (iXon Ultra 888; 1024×1024 pixels)
  • Image acquisition: NIS-Elements AR and Home-built software

장비 사용 문의

  • 장비 위치: 서울대 융합과학기술대학원(광교) D동 B106호
  • 담당자: 김정민 (jeomik@snu.ac.kr, (+82) 31-888-9082)
  • 공용 장비 운용 (2021년 4월~)
  • 장비 사용료 안내

Lasers

  • Laser diode system: 405 nm CW, 50 mW (OBIS 405 LX 50 mW, Coherent)
  • DPSS laser: 488 nm CW, 200 mW (Sapphire 488 HP, Coherent)
  • Fiber laser: 560 nm CW, 1.0 W (2RU-VFL-P-1000-560-B1R, MPB Communications)
  • Fiber laser: 642 nm CW, 1.0 W (2RU-VFL-P-1000-642-B1R, MPB Communications)
  • Ti:Sapphire femtosecond laser: (to be updated)

High-end cameras

Optical modulators

  • AOTF (97-03151-01, G&H): CA 2.5 mm, 400-650 nm, 55-110 MHz
  • SLM (PLUTO-2-VIS-096, Holoeye Photonics AG): Reflective LCOS (phase only), 1920×1080 pixels, 8 µm pixel pitch, ~2π phase range (@650 nm; 8bit), 95~98% reflectivity (dielectric mirror coating, 450-650 nm)

Optical scanner

  • Galvanometer scanner: non-resonant (to be updated)

Precision Actuator (Piezo)

  • PIFOC Objective scanning system (PD72Z2CA0, Physik Instrumente): 250 µm travel, 1.5 nm resolution (closed loop)
  • PIFOC Objective scanning systems (Physik Instrumente): 400 µm travel, (more spec to be updated)

Microscope objective lens

High-end objectives!
  • 100x/1.45, oil immersion (Nikon CFI Plan Apo Lambda 100x oil)
  • 100x/1.49 TIRF, oil immersion (Nikon CFI SR HP Apo TIRF 100xc oil)
  • 100x/1.35, silicone oil immersion (Nikon CFI SR HP Plan Apo Lambda S 100xc sil)
  • 60x/1.27, water immersion (Nikon CFI SR Plan Apo IR 60xc WI)
  • 100x/1.45 (inf/0.17), Olympus UPLXAPO100XO (to be added)
  • 100x/1.4 (inf/0.17), Olympus UPLSAPO100XO
  • 50x/0.95 (inf/0), Olympus MPlanApo50x
  • 20x/0.75 (inf/0.17), Nikon CFI Plan Apo Lambda
  • 20x/0.40 (inf/0), Olympus LMPlanFl

Instrument

  • Hand-held refractometer (PAL-RI, Atago): refractive index from 1.3306 to 1.5284
  • Optical Microscopy Educational Kit (EDU-OMC1/M, Thorlabs)
  • Compact spectrometer (CCS200/M, Thorlabs): 200-1000 nm (wavelength range), <2 nm FWHM (@633 nm, spectral accuracy)