Multi-functional optical imaging system (다기능성 광학 이미징 시스템)
이미징 모드
- 광시야 형광 이미징 (Conventional fluorescence microscopy)
- TIRF (Total Internal Reflection Fluorescence) Microscopy
- SMLM (Single Molecule Localization Microscopy)
– STORM: 25/50 nm (횡방향/종방향 분해능) for organic dyes
– PALM: 40/100 nm (횡방향/종방향 분해능) for fluorescent proteins
– PAINT (Point Accumulation for Imaigng in Nanoscale Topography)
사양
- Microscope platform: Nikon’s Ti2-E
- Laser lines: 405 nm, 488 nm, 560 nm, 642 nm
- Objectives: 100x/1.45 (oil), 100x/1.49 (oil, TIRF), 100x/1.35 (silicone oil), 60x/1.27 water, 20x/0.75
- Cameras: Andor’s EMCCD (iXon Ultra 888; 1024*1024 pixels)
- Image acquisition: NIS-Elements AR and Home-built software
장비 사용 문의
- 장비 위치: 서울대 융합과학기술대학원(광교) D동 B106호
- 담당자: 김정민 (jeomik@snu.ac.kr, (+82) 31-888-9082)
- 공용 장비 운용 (2021년 4월~)
장비 사용료 안내
Lasers
- Laser diode system: 405 nm CW, 50 mW (OBIS 405 LX 50 mW, Coherent)
- DPSS laser: 488 nm CW, 200 mW (Sapphire 488 HP, Coherent)
- Fiber laser: 560 nm CW, 1.0 W (2RU-VFL-P-1000-560-B1R, MPB Communications)
- Fiber laser: 642 nm CW, 1.0 W (2RU-VFL-P-1000-642-B1R, MPB Communications)
High-end cameras
- sCMOS: 2048×2048 pixels, max QE > 95% (back illuminated), Andor’s Sona 4.2B-6 (SONA-4BV6U)
- EMCCD: 1024×1024 pixels, Andor’s iXon Ultra 888 (DU-888U3-CS0-BV)
Optical modulators
- AOTF: CA 2.5 mm, 400-650 nm, 55-110 MHz (97-03151-01, G&H)
- SLM: Reflective LCOS (phase only), 1920×1080 pixels, PLUTO-2-VIS-096, Holoeye Photonics AG)
Microscope objective lens
- 100x/1.45, oil immersion (Nikon CFI Plan Apo Lambda 100x oil)
- 100x/1.49 TIRF, oil immersion (Nikon CFI SR HP Apo TIRF 100xc oil)
- 100x/1.35, silicone oil immersion (Nikon CFI SR HP Plan Apo Lambda S 100xc sil)
- 60x/1.27, water immersion (Nikon CFI SR Plan Apo IR 60xc WI)
- 100x/1.45 (inf/0.17), Olympus UPLXAPO100XO (to be added)
- 100x/1.4 (inf/0.17), Olympus UPLSAPO100XO
- 50x/0.95 (inf/0), Olympus MPlanApo50x
- 20x/0.75 (inf/0.17), Nikon CFI Plan Apo Lambda
- 20x/0.40 (inf/0), Olympus LMPlanFl
Instrument
- Hand-held refractometer (Atago, PAL-RI)
- Optical Microscopy Educational Kit (EDU-OMC1/M, Thorlabs)